Altered circulating concentrations of active glucagon-like peptide (GLP-1) and dipeptidyl peptidase 4 (DPP4) in obese subjects and their association with insulin resistance

作者：Ahmed Radwan H; Huri Hasniza Zaman; Muniandy Sekaran^*; Al Hamodi Zaid; Al absi Boshra; Alsalahi Abdulsamad; Razif Muhammad F M^*

来源：Clinical Biochemistry, 2017, 50(13-14): 746-749.

DOI：10.1016/j.clinbiochem.2017.03.008

• Obesity
• Insulin resistance
• Dipeptidyl peptidase 4
• sDPP4
• GLP-1

Objectives: Soluble DPP4 (sDPP4) is a novel adipokine that degrades glucagon-like peptide (GLP-1). We evaluated the fasting serum levels of active GLP-1 and sDPP4 in obese, overweight and normal weight subjects to assess the association between sDPP4 levels, active GLP-1 levels and insulin resistance in obese subjects.
Methods: The study involved 235 Malaysian subjects who were randomly selected (66 normal weight subjects, 97 overweight, 59 obese subjects, and 13 subjects who were underweight). Serum sDPP4 and active GLP-1 levels were examined by enzyme-linked immunosorbent assay (ELISA). Also, body mass index kg/m(2) (BMI), lipid profiles, insulin and glucose levels were evaluated. Insulin resistance (IR) was estimated via the homeostasis model assessment for insulin resistance (HOMA-IR).
Results: Serum sDPP4 levels were significantly higher in obese subjects compared to normal weight subjects (p = 0.034), whereas serum levels of active GLP-1 were lower (p = 0.021). In obese subjects, sDPP4 levels correlated negatively with active GLP-1 levels (r(2) = -0.326, p = 0.015). Furthermore, linear regression showed that sDPP4 levels were positively associated with insulin resistance (B = 82.28, p = 0.023) in obese subjects.
Conclusion: Elevated serum sDPP4 levels and reduced GLP-1 levels were observed in obese subjects. In addition, sDPP4 levels correlated negatively with active GLP-1 levels but was positively associated with insulin resistance. This finding provides evidence that sDPP4 and GLP-1 may play an important role in the pathogenesis of obesity, suggesting that sDPP4 may be valuable as an early marker for the augmented risk of obesity and insulin resistance.

• 出版日期2017-9