Antibody immobilization on a nanoporous aluminum surface for immunosensor development

作者：Chai Changhoon^*; Lee Jooyoung; Park Jiyong; Takhistov Paul

来源：Applied Surface Science, 2012, 263: 195-201.

DOI：10.1016/j.apsusc.2012.09.027

• Immunosensor
• Antibody immobilization
• Electrochemical impedance spectroscopy
• Nanoporous structure
• Aluminum anodization
• Silanization
• 3-Aminopropyltryethoxysilane (APTES)

A method of antibody (Ab) immobilization on a nanoporous aluminum surface for an electrochemical immunosensor is presented. To achieve good attachment and stability of Ab on an aluminum surface, aluminum was silanized with 3-aminopropyltryethoxysilane (APTES), and then covalently cross-linked to self-assembled layers (SALs) of APTES. Both the APTES concentration and the silanization time affected the formation of APTES-SALs as Ab immobilization. The formation of APTES-SALs was confirmed using the water contact angle on the APTES-SALs surface. The reactivity of APTES-SALs with Ab was investigated by measuring the fluorescence intensity of fluorescein isothiocyanate-labeled Ab-immobilized on the aluminum surface. Silanization of aluminum in 2% APTES for 4 h resulted in higher water contact angles and greater amounts of immobilized Ab than other APTES concentrations or silanization times. More Ab was immobilized on the nanoporous surface than on a planar aluminum surface. Electrochemical immunosensors developed on the nanoporous aluminum via the Ab immobilization method established in this study responded functionally to the antigen concentration in the diagnostic solution.

• 出版日期2012-12-15