Database Searching in Mass Spectrometry Based Proteomics

作者：Kertesz Farkas Attila^*; Reiz Beata; Myers Michael P; Pongor Sandor

来源：Current Bioinformatics, 2012, 7(2): 221-230.

DOI：10.2174/157489312800604354

• Database search
• Mass spectrometry
• Protein identification
• Proteomics

Bottom-up proteomics (mass spectrometry analysis of peptides obtained by proteolysis and separated by liquid chromatography, (LC-MS/MS)) is one of the most frequently used techniques for identifying and characterizing proteins in biological samples. A key element of the analysis is database searching when the mass spectra of the peptides are compared with a database of theoretically computed (or experimental) peptide spectra. Here we discuss the main computational approaches to spectrum database searching and the statistical analysis of the results.

• 出版日期2012-6