ANTI-INFLAMMATORY EFFECT AND MECHANISM OF ACTION OF LINDERA ERYTHROCARPA ESSENTIAL OIL IN LIPOPOLYSACCHARIDE-STIMULATED RAW264.7 CELLS

作者：Ko Yeong Jong; Ahn Ginnae; Ham Young Min; Song Sang Mock; Ko Eun Yi; Cho Su Hyeon; Yoon Weon Jong^*; Kim Kil Nam^*

来源：EXCLI Journal, 2017, 16: 1103-1113.

DOI：10.17179/excli2017-596

• Lindera erythrocarpa
• anti-inflammatory
• essential oil
• NF-kappa B
• MAPK

The aim of this study was to investigate the chemical constituents of Lindera erythrocarpa essential oil (LEO) by gas chromatography-mass spectrometry and evaluate their inhibitory effect on the expression of pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Fifteen compounds, accounting for 63.7 % of the composition of LEO, were identified. The main compounds were nerolidol (18.73 %), caryophyllene (14.41 %), alpha-humulene (7.73 %), germacrene-D (4.82 %), and a-pinene (4.47 %). LEO significantly inhibited the expression of inducible nitric oxide (NO) synthase and cyclooxygenase-2, and subsequent production of NO and prostaglandin E-2. In addition, it reduced the release of pro-inflammatory cytokines in LPS-activated RAW264.7 cells. The molecular mechanism underlying the effect of LEO was associated with inhibition of the phosphorylation of mitogen-activated protein kinase (MAPK). Furthermore, LEO inhibited LPS-induced phosphorylation and degradation of inhibitor of kappa B-alpha, which is required for the activation of the p50 and p65 nuclear factor (NF)-kappa B subunits in RAW264.7 cells. Taken together, these data suggest that LEO exerted its anti-inflammatory effect by downregulating LPS-induced production of pro-inflammatory mediators through the inhibition of NF-kappa B and MAPK signaling in RAW264.7 cells.

• 出版日期2017