Netrin-4 Promotes Differentiation and Migration of Osteoblasts

作者：Enoki Yuichiro; Sato Tsuyoshi^*; Kokabu Shoichiro; Hayashi Naoki; Iwata Takanori; Yamato Masayuki; Usui Michihiko; Matsumoto Masahito; Tomoda Taketo; Ariyoshi Wataru; Nishihara Tatsuji; Yoda Tetsuya

来源：In Vivo, 2017, 31(5): 793-799.

DOI：10.21873/invivo.11132

• Netrin-4
• osteoblast
• differentiation
• migration

Background/Aim: While netrin-4 plays a vital role in the vascular system, the role of netrin-1 in osteoblast differentiation is not well understood. In this study we explored whether netrin-4 has functional roles in osteoblasts. Materials and Methods: Quantitative reverse-transcriptase polymerase chain reaction (PCR), RNA interference, the generation of plasmids, transfections, measurement of alkaline phosphatase activity, a mineralization assay, a migration assay and a cell proliferation assay were performed. Results: Netrin-4 expression was up-regulated during osteoblast differentiation and an RNA interference experiment showed that small interfering RNA used to silence netrin-4 inhibited osteoblast differentiation. Recombinant mouse netrin-4 promoted alkaline phosphatase (ALP) activity of osteoblasts and enhancement of calcium deposits. Moreover, we constructed a vector containing the netrin-4 gene on the basis of the plasmid pcDNA3.1/V5-His. Overexpression of netrin-4 enhanced differentiation of osteoblasts. Finally, recombinant mouse netrin-4 promoted cell migration of osteoblasts. Conclusion: Netrin-4 promotes differentiation and migration of osteoblasts.

• 出版日期2017-10