A bioluminescence assay for aldehyde dehydrogenase activity

作者：Duellman Sarah J^*; Valley Michael P; Kotraiah Vinayaka; Vidugiriene Jolanta; Zhou Wenhui; Bernad Laurent; Osterman Jean; Kimball Joshua J; Meisenheimer Poncho; Cali James J

来源：Analytical Biochemistry, 2013, 434(2): 226-232.

DOI：10.1016/j.ab.2012.11.016

• Bioluminescent aldehyde dehydrogenase assay
• Bioluminescence
• Luciferase
• High-throughput screening

The aldehyde dehydrogenase (ALDH) family of enzymes is critical for cell survival and adaptation to cellular and environmental stress. These enzymes are of interest as therapeutic targets and as biomarkers of stem cells. This article describes a novel, homogeneous bioluminescence assay to study the activity of the ALDH enzymes. The assay is based on a proluciferin-aldehyde substrate that is recognized and utilized by multiple ALDH enzyme isoforms to generate luciferin. A detection reagent is added to inactivate ALDH and generate light from the luciferin product. The luminescent signal is dependent on the ALDH enzyme concentration and the incubation time in the ALDH reaction; moreover, the luminescent signal generated with the detection reagent is stable for greater than 2 h. This assay provides many advantages over standard NADH fluorescence assays. It is more sensitive and the signal stability provided allows convenient assay setup in batch mode-based high-throughput screens. The assay also shows an accurate pharmacological response for a common ALDH inhibitor and is robust, with a large assay window (S/B = 64) and Z' = 0.75.

• 出版日期2013-3-15