A likely role for the PH-domain containing protein, PEPP2/PLEKHA5, at the membrane-microtubule cytoskeleton interface

作者：Zou Yi; Cox Timothy C^*

来源：Biocell, 2013, 37(3): 55-61.

DOI：10.32604/biocell.2013.37.055

• microtubule
• membrane
• phosphoinositide
• microviscosity
• PH domain

PH (pleckstrin homology) domains are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular compartments with assistances of alternative binding partners. PH domain-containing proteins have been found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2 (also known as PLEKHA5), displays moderate phosphoinositide binding specificity. Full length PEPP2 was observed to variably associate with both the plasma membrane and microtubules. The membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating cells. Overexpression of PEPP2 increased membrane microviscosity, indicating a potential role for PEPP2 in regulating function of microtubule-dependent membrane functions.

• 出版日期2013