科研之友
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摘要
The present study aims to evaluate the antiparasitic activity of active components from Cynanchum paniculatum against Ichthyophthirius multifiliis. The antiparasitic activities of two bioassay-guided fractionationated compounds from C. paniculatum identified as Cynatratoside-A and Cynanversicoside C, by comparing spectral data (NMR and ESI-MS) with literature values, were evaluated by in vitro assay. These showed that both could kill theronts of I. multifiliis at a concentration of 10.0 mg L-1, with the median effective concentration (EC50) values of 4.6 mg L-1 and 5.2 mg L-1 for Cynatratoside-A and Cynanversicoside C, respectively. Encysted tomonts were killed at concentrations of 8.0 mg L-1 with both compounds. In vivo experiments demonstrated that fish treated with both compounds at 15.0 mg L-1 carried significantly fewer parasites than controls (P < 0.05). There were no mortalities among treated fish group compared with 75% mortality of untreated fish. Cynatratoside-A and Cynanversicoside C are therefore potential candidate drugs for use against I. multifiliis.
