Applied scientific papers presented exclusively for you Detection of plant contamination in food by real-time PCR

作者：Focke Felix^*; Haase Ilka; Fischer Markus

来源：Deutsche Lebensmittel-Rundschau, 2011, 107(5): 221-224.

Contaminations of food with toxic plant material can cause severe health problems. For instance, ingestion of Senecio species should be avoided due to the content of toxic pyrrolizidine alkaloids. On the other hand, some plant derived ingredients can lead to allergic reactions which can be life-threatening for sensitive persons. Generally, plant contaminations can be detected via DNA-based techniques. A conventional PCR method for the detection of spices and herbs in food was adapted to real-time conditions to detect common groundsel (Senecio vulgaris) in rocket salad. It could be shown that by the real-time PCR method S. vulgaris can be reliably detected in a range from 1 ppm up to 10 %.
Furthermore the described methods to detect mustard (Sinapis alba and Brassica nigra) and celery (A plum graveolens) were also adapted to real-time conditions. It could be shown that the developed methods are able to detect those plants even in low ppm ranges.

• 出版日期2011-5