科研之友
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摘要
Chloride (Cl-) plays an important role in many cellular responses, including control of membrane potential, neurotransmission, regulation of cell volume and charge balance. Due to its physiological relevance, there is a growing need for improved optical sensors that can detect Cl- ions in biological and environmental samples. Herein, we have developed new N-acylhydrazone substituted BODIPY-mercury(II) based fluorescent turn-on sensors 1-Hg2+ and 2-Hg2+, which showed remarkable selectivity and specificity towards Cl- ions under physiological conditions. The new BODIPY compounds 1 and 2 were synthesized by treating their corresponding 3,5-diformyl BODIPYs 3 and 4 with isonicotinohydrazide in one step under simple reaction conditions. Compounds 1 and 2 absorb in 615-650 nm and emit in 625-660 nm regions. BODIPYs 1 and 2 showed exclusive sensing towards Hg2+ and formed 1-Hg2+ and 2-Hg2+ complexes, respectively, which resulted in the quenching of fluorescence. Job%26apos;s plot analyses and HR-MS studies supported the formation of 1-Hg2+ and 2-Hg2+ complexes. Complexes 1-Hg2+ and 2-Hg2+ were found to be exclusive fluorescence turn-on sensors for chloride ions. Upon addition of Cl- ions to 1-Hg2+ and 2-Hg2+ complexes, the mercury(II) ions were extracted from the complexes by releasing the free BODIPYs 1 and 2 which reflected in the significant enhancement of fluorescence intensity (22-fold) with a detection limit of 108 nM. The reversibility and reusability of sensors for the detection of Hg2+ and Cl- ions were tested for six cycles. Interestingly, the sensor can be used to construct an IMPLIES logic gate system as demonstrated in this paper. Furthermore, the probe is cell membrane-permeable and can readily be used to detect the intracellular Cl- ions.
- 出版日期2014
