NF-kappa B p65 represses beta-catenin-activated transcription of cyclin D1

作者：Hwang Injoo; Choi Yong Seok; Jeon Mi Ya; Jeong Sunjoo^*

来源：Biochemical and Biophysical Research Communications, 2010, 403(1): 79-84.

DOI：10.1016/j.bbrc.2010.10.118

• beta-catenin
• TCF
• NF-kappa B
• Cyclin D1

Signaling crosstalk between the beta-catenin and NF-kappa B pathways represents a functional network. To test whether the crosstalk also occurs on their common target genes, the cyclin D1 promoter was used as a model because it contains binding sites for both proteins. beta-catenin activated transcription from the cyclin D1 promoter, while co-expression of NF-kappa B p65 reduced beta-catenin-induced transcription. Chromatin immunoprecipitation revealed lithium chloride-induced binding of beta-catenin on one of the T-cell activating factor binding sites. More interestingly, beta-catenin binding was greatly reduced by NF-kappa B p65, possibly by the protein-protein interaction between the two proteins. Such a dynamic and complex binding of beta-catenin and NF-kappa B on promoters might contribute to the regulated expression of their target genes.

• 出版日期2010-12-3