Hyaluronate - parathyroid hormone peptide conjugate for transdermal treatment of osteoporosis

作者：Cho Minsoo; Han Seulgi; Kim Hyemin; Kim Ki Su; Hahn Sei Kwang

来源：Journal of Biomaterials Science, Polymer Edition, 2018, 29(7-9): 793-804.

DOI：10.1080/09205063.2017.1399001

• Human parathyroid hormone
• hyaluronic acid
• transdermal delivery
• osteoporosis

Human parathyroid hormone 1-34 fragment (PTH1-34) has been used as a FDA-approved therapeutics to treat osteoporosis by daily subcutaneous injection. In this work, we successfully developed PTH1-34 conjugated hyaluronic acid (HA) for the transdermal treatment of osteoporosis with improved patient compliance. HA-PTH1-34 conjugate was synthesized by the coupling reaction between aldehyde group introduced to HA and amine group of PTH1-34. After characterization by gel permeation chromatography (GPC) and ELISA, the biological effect of HA-PTH1-34 conjugate on the proliferation of human osteoblast cells was confirmed by in vitro calcium colorimetric assay and cAMP assay. Two-photon microscopy clearly visualized the effective skin penetration of FITC modified HA-PTH1-34 conjugate. The transdermally delivered HA-PTH1-34 conjugates elevated the blood calcium concentration for more than 2days, reflecting the feasibility for the treatment of osteoporosis.

• 出版日期2018