A novel lipid compound, epolactaene, induces apoptosis: its action is modulated by its side chain structure

作者：Nakai J; Kawada K; Nagata S; Kuramochi K; Uchiro H; Kobayashi S; Ikekita M^*

来源：Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids, 2002, 1581(1-2): 1-10.

DOI：10.1016/S1388-1981(01)00169-X

• epolactaene
• leukemia B cell line
• cell death
• apoptosis
• neurite outgrowth
• side chain structure

A novel lipid compound, epolactaene, was isolated from the culture supernatant of Penicilllum sp. 1689-P and it has already been reported that it induced neurite outgrowth in a human neuroblastoma cell line. In this study, we first investigated the effects of epolactaene on a human leukemia B-cell line, BALL-1 cells, and clarified that epolactaene induces apoptosis in BALL-1 cells in a dose- and tithe-dependent manner. Furthermore, we focused on the side chain structure of epolactaene, and chemically synthesized epolactaene derivatives. One derivative, which has a straight long alkyl chain as its side chain, induced apoptosis more effectively than epolactaene. On the other hand, other derivatives with a short alkyl side chain had weaker apoptosis-inducing actions. A good correlation was found between the apoptosis-inducing action of these compounds and their octanol/water partition coefficients (log P). These results suggested that the apoptosis-inducing activities of epolactaene and its derivatives were related to the hydrophobicity of these compounds; so that side chain structure of epolactaene is very important for its apoptosis-inducing activities. These apoptosis-inducing actions of epolactaene and its derivatives were also observed in various blood tumor cell lines and normal lymphocytes.

• 出版日期2002-3-15