科研之友
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摘要
To understand the pathogenesis of cervical cancer (CC) associated with polarity protein alpha PKC and the potential roles of alpha PKC in clinical management of CC. Tissue samples were collected from women who received colposcopy biopsy or hysterectomy surgery, including 9 CIN1, 8 CIN2, 15 CIN3, and 12 invasive cervical squamous cancer (ICC). 16 normal controls were from the normal region of tumor samples, HE and immunofluorescence staining of alpha PKC were performed on these samples. ANOVA and Kruslal-wallis test were used to quantitate the abnormal distribution and expression level of alpha PKC among different cervical lesions. Disruption of polarized apical localization and increased cytoplasmic accumulation of alpha PKC were identified in cervical lesions. In normal cervical epithelium, alpha PKC was detected on the apical membrane of endocervical columnar epithelial cells and of exocervical epithelial cells located at basal layer of squamous epithelium. While in squamous metaplasia, a precancerous lesion of cervical neoplasia, the polarized apical membrane localization of alpha PKC was disrupted, and intensed cytoplasmic accumulation was identified in the immature squamous metaplastic cells. Compared with normal cervix, number of epithelial cells with abnormal alpha PKC distribution was progressively increased in CINs and ICC (P < 0.05), and cytoplasmic accumulation of alpha PKC was increased in CIN2, CIN3, and ICC compared with CIN1 (P < 0.05). Disruption of polarized apical localization and increased cytoplasmic accumulation of alpha PKC were associated with CC progression, indicating that precise regulation of alpha PKC may play important roles in CC progression, and alpha PKC may be a potential molecular target for clinical diagnoses and treatment of CC.
- 出版日期2013-8
- 单位四川大学
