Determination of Monosaccharide Constituents in Lycium Barbarum Polysaccharide Using Capillary Ion Chromatography with Pulsed Amperometric Detection

作者：Li Jing^*; Li Ren Yong; Liang Li Na

来源：Chinese Journal of Analytical Chemistry, 2012, 40(9): 1415-1420.

DOI：10.3724/SP.J.1096.2012.20061

• Capillary ion chromatography
• Pulsed amperometric detection
• Lycium barbarum polysaccharide
• Monosaccharide

A method for the determination of ten kinds of monosaccharide in Lycium barbarum polysaccharide was established using ICS-5000 Capillary IC system. In the pretreatment process, the type and concentration of acid, the hydrolysis temperature and lasting time were selected or optimized. The optimized hydrolysis condition was 100 oC lasting 120 min with 2 mol/L trifluoroacetic acid solution. Under this condition the recovery rate of fructose was about 50%. Recovery rate of the rest monosaccharide was 84% to 104%. In the experiment, new type of Eluent Generator, Capillary CarborPac PA20 column, and improved capillary amperometric detection cell were used. The calibration curves of ten kinds of monosaccharide showed good linearity with correlation coefficient above 0.999. The LOD is between 2.5 to75 mu g/L. It is suitable for the determination of monosaccharide constituents in Lycium barbarum polysaccharide.

• 出版日期2012-9