Size and site dependent biological hazard potential of particulate matters collected from different heights at the vicinity of a building construction

作者：Li Huaqiong; Fang Crystal Hay Yu; Shi Wenxiong; Gurusamy Subramaniam; Li Shuzhou; Krishnan Manoj N; George Saji^*

来源：Toxicology Letters, 2015, 238(3): 20-29.

DOI：10.1016/j.toxlet.2015.08.002

• Building-construction
• Particulate matter
• Risk assessment
• Cytotoxicity
• BEAS-2B
• RAW 264.7

Although building constructions are a recurring part of urbanization, the health risk of particulate matters (PM) originating from such activities have seldom been subjected to detailed studies. We sought to characterize the relative risk of air borne PM collected from different heights (ground and top floor) of a building adjacent to a building under early phase of construction. We determined the physico-chemical properties such as size and shape, elemental composition and surface charge of the PM. The oxidative stress dependent cytotoxic and pro-inflammatory responses were assessed in BEAS-2B and RAW 264.7 cell lines using high-content-screening platforms. In comparison to top floor, the total mass of PM collected from ground floor was two-three folds higher and the mass fraction was dominated by PM20-35. Elemental analysis showed abundance of Si, Al, K, Ca and Fe in bigger PM while for PM0.25-0.5 it was mostly constituted by C and crystals rich in S and K. PM caused NF kappa B activation, secretion of pro-inflammatory cytokines and cytotoxicity wherein PM0.25-0.5 was the most potent among the tested PM. Estimated exposure level and lung burden together with the data on hazard potential were used for

• 出版日期2015-11-4