Aged Garlic Extract Attenuates Cerebral Damage and Cyclooxygenase-2 Induction after Ischemia and Reperfusion in Rats

作者：Laura Colin Gonzalez Ana; Ortiz Plata Alma; Villeda Hernandez Juana; Barrera Diana; Molina Jijon Eduardo; Pedraza Chaverri Jose; Maldonado Perla D^*

来源：Plant Foods for Human Nutrition, 2011, 66(4): 348-354.

DOI：10.1007/s11130-011-0251-3

• Aged garlic extract
• Cerebral ischemia
• TNF alpha
• Cyclooxygenase-2
• Inflammation
• Oxidative stress

Different garlic products reduce the cerebral ischemic damage due to their antioxidant properties. In this work, we investigated the effect of aged garlic extract (AGE) on cyclooxygenase-2 (COX-2) protein levels and activity, and its role as a possible mechanism of neuroprotection in a cerebral ischemia model. Animals were subjected to 1 h of ischemia plus 24 h of reperfusion. AGE (1.2 ml/kg weight, i.p.) was administered at onset of reperfusion. To evaluate the damage induced by cerebral ischemia, the neurological deficit, the infarct area, and the histological alterations were measured. As an oxidative stress marker to deoxyribonucleic acid, 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were determined. Finally, as inflammatory markers, TNF alpha levels and COX-2 protein levels and activity were measured. AGE treatment diminished the neurological alterations (61.6%), the infarct area (54.8%) and the histological damage (37.7%) induced by cerebral ischemia. AGE administration attenuated the increase in 8-OHdG levels (77.8%), in TNF alpha levels (76.6%), and in COX-2 protein levels (73.6%) and activity (30.7%) induced after 1 h of ischemia plus 24 h of reperfusion. These data suggest that the neuroprotective effect of AGE is associated not only to its antioxidant properties, but also with its capacity to diminish the increase in TNF alpha levels and COX-2 protein expression and activity. AGE may have the potential to attenuate the cerebral ischemia-induced inflammation.

• 出版日期2011-11