Brain tryptophan rather than pH-value is altered as consequence of artificial postmortem interval and storage conditions

作者：Gruenblatt Edna; Proft Florian; Apfelbacher Manuela; Deckert Juergen; Roggendorf Wolfgang; Riederer Peter; Monoranu Camelia Maria

来源：Neurochemistry International, 2010, 57(7): 819-822.

DOI：10.1016/j.neuint.2010.08.020

• Brain
• Postmortem
• Tryptophan
• pH-value
• Postmortem interval
• Temperature

Brain bank centers around the world attempt to standardize postmortem brain collection and quality control. Antemortem as well postmortem factors may influence tissue quality. Previously, we could demonstrate that increased tryptophan (TRP) levels significantly correlate to prolonged postmortem interval (PMI) and storage duration, whereas pH-value altered merely as consequence of prolonged agonal state and ischemic brain damage additionally to repeated freeze and thaw cycles. Therefore, we aimed to investigate in artificial PMI conditions, with three brain tissue storage temperatures (4 degrees C, room temperature and 37 degrees C) as well as oxidizing conditions (open/close tube), whether TRP levels and pH-value alter. We could confirm that prolonged PMI at higher storage temperatures and oxidizing conditions significantly correlate to increased TRP levels, while pH-value did not correlate at all. In conclusion, from these results PMI intervals until autopsy should be kept as short as possible and storage until autopsy should be at 4 degrees C in order to preserve brain tissue quality as much as possible.

• 出版日期2010-12