A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

作者:Chandrasekaran Aarthi*; Deng Kai; Koh Chung Yan; Takasuka Taichi; Bergeman Lai F; Fox Brian G; Adams Paul D; Singh Anup K
来源:Chemical Communications, 2013, 49(48): 5441-5443.
DOI:10.1039/c3cc39155h

摘要

We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.

  • 出版日期2013