Optimization of the selective preparation of 20(R)-ginsenoside Rg3 catalyzed by D, L-tartaric acid using response surface methodology

作者：Sun, Chengpeng; Gao, Weiping; Zhao, Baozhong; Cheng, Leqin^*

来源：Fitoterapia, 2013, 84: 213-221.

DOI：10.1016/j.fitote.2012.11.011

• Hydrolysis
• Selective preparation
• 20(R)-ginsenoside Rg3
• Response surface methodology
• Protopanaxadiol type saponins

The optimization of the selective preparation of 20(R)-ginsenoside Rg3 converting protopanaxadiol type saponins (PPD saponins) by the commercially available D, L-tartaric add was carried out using response surface methodology (RSM) based on a three-factor and six-level central composite design. The optimal 20(R)-ginsenoside Rg3 de% was predicted to be 94.52% in the combination of the factors (D, L-tartaric acid concentration 1.19 mol/L, temperature 107.9 degrees C and time 2.79 h) through the canonical analysis with maximum responses. Under the optimum reaction conditions, the actual 20(R)-ginsenoside Rg3 de% was 96.49%. 20(R)-ginsenoside Rg3 (1) and 20(S)-ginsenoside Rg3 (2) were separated and identified by H-1-NMR and C-13-NMR. Therefore, the RSM was effective to optimize the preparation of 20(R)-ginsenoside Rg3 by converting PPD saponins using D, L-tartaric acid.

• 出版日期2013-1
• 单位东北师范大学; 吉林化工学院