A novel animal model for studying pancreatic regeneration by employing photochemical reaction

作者:Han Shengjun; Joo Kwang Ro*; Jeon Jung Won; Lim Jun Uk; Park Jae Jun; Shin Hyun Phil; Cha Jae Myung; Huh Youngbuhm
来源:Pancreatology, 2012, 12(1): 74-78.
DOI:10.1016/j.pan.2011.11.004

摘要

Purpose: We present photochemical-induced pancreatic necrosis (PIPN) as a novel induction method for studying pancreatic regeneration in an animal model. %26lt;br%26gt;Methods: Photosensitive Rose Bengal was injected through the femoral vein in rats, followed by illumination of the surface of the pancreas with a cool halogen light for a period of 20 min. At 3, 6, and 24 h, and 7, 10, 14, and 20 days, experimental animals were sacrificed; all the animals received intravenous injection with 5-bromo-2-deoxyuridine (BrdU) 1 h prior to sacrifice. %26lt;br%26gt;Results: At 3-6 h of induction of PIPN, pancreatic necrosis was superficially observed in the illuminated field. At 24 h, there was a slight increase in the depth and width of the lesion along with appearance of vascular congestion and thrombosis in the lesion. On days 7-10, the area of illumination was totally replaced by necrotic pancreatic tissue, inflammatory cell infiltrates, and newly appearing cellular components, including mesenchymal and epithelial cells, which formed tubular complexes. On day 14, clusters of tubular complexes intermingled with acinar cells, which were proven as newly formed acinar tissue by BrdU staining. On day 20, all the lesions had returned to a normal state of pancreatic tissue. %26lt;br%26gt;Conclusion: This study demonstrates the potential of PIPN as a valuable method for production of an animal model for studying healing processes or regeneration of pancreatic tissue after injury.

  • 出版日期2012-2

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