Activin A can induce definitive endoderm differentiation from human parthenogenetic embryonic stem cells

作者：Wang, Zhiqiang; Li, Wenliang; Chen, Tianxing; Yang, Jun; Wen, Zhengqi; Yan, Xinmin; Shen, Tao; Liang, Rui^*

来源：Biotechnology Letters, 2015, 37(8): 1711-1717.

DOI：10.1007/s10529-015-1829-x

• Activin A
• Definitive endoderm
• Differentiation
• Human foreskin fibroblasts
• Human parthenogenetic embryonic stem cells
• Stem cells

As activin/nodal signaling plays a key role in definitive endoderm (DE) differentiation, we have explored activin A-induced differentiation of DE from human parthenogenetic embryonic stem cells (hPESCs).
Administration of 5 ng activin A/ml had no effect on the expression of markers of DE differentiation. However, higher concentrations of activin A (50 and 100 ng/ml) upregulated Sox17 and Cxcr4, as well upregulating the mesendodermal precursor marker, Brachyury.
These findings demonstrate that low dose activin A can maintain the undifferentiated potency of hPESCs, whereas higher doses induce DE differentiation; 50 ng/ml is the optimal concentration for inducing DE from hPESCs.

• 出版日期2015-8
• 单位云南省第一人民医院; 昆明医科大学