A role for AMPK in the inhibition of glucose-6-phosphate dehydrogenase by polyunsaturated fatty acids

作者：Kohan Alison B; Talukdar Indrani; Walsh Callee M; Salati Lisa M^*

来源：Biochemical and Biophysical Research Communications, 2009, 388(1): 117-121.

DOI：10.1016/j.bbrc.2009.07.130

• Polyunsaturated fatty acids
• Lipogenesis
• Primary hepatocytes
• AMP-activated protein kinase

Both polyunsaturated fatty acids and AMPK promote energy partitioning away from energy consuming processes, such as fatty acid synthesis, towards energy generating processes, such as beta-oxidation. In this report, we demonstrate that arachidonic acid activates AMPK in primary rat hepatocytes, and that this effect is p38 MAPK-dependent. Activation of AMPK mimics the inhibition by arachidonic acid of the insulin-mediated induction of G6PD. Similar to intracellular signaling by arachidonic acid, AMPK decreases insulin signal transduction, increasing Ser(307) phosphorylation of IRS-1 and a subsequent decrease in AKT phosphorylation. Overexpression of dominant-negative AMPK abolishes the effect of arachidonic acid on G6PD expression. These data suggest a role for AMPK in the inhibition of G6PD by polyunsaturated fatty acids.

• 出版日期2009-10-9
• 单位河北医科大学