Neuroglobin upregulation offers neuroprotection in traumatic brain injury

作者：Shang, Aijia; Feng, Xingjun; Wang, Hangyan; Wang, Jing; Hang, Xingyi; Yang, Ying; Wang, Zhongfeng; Zhou, Dingbiao^*

来源：Neurological Research, 2012, 34(6): 588-594.

DOI：10.1179/1743132812Y.0000000052

• Traumatic brain injury
• Neuroglobin
• Upregulation
• Adenoviral vectors
• Neuroprotection

Objectives: The aim of this study was to investigate rat neuroglobin (rNGB) expression level after traumatic brain injury (TBI) and further study its neuroprotective effects in TBI when it was overexpressed in adenoviral vector.
Methods: The Wistar rats (280-320 g) were divided into control, 12 and 36 hours after TBI groups (n = 3) and their TBI model was established. Subsequently, NGB expression level was examined by Western blot and immunohistochemical. Beyond that, adenoviral vectors pAdEasy-rNGB-GFP and pAdEasy-GFP were constructed and transfected into the rat brain respectively (pAdEasy-GFP was control), and the neuroprotective effects were examined by immunohistochemical.
Results: Immunohistochemical and Western blot results demonstrated that NGB expression level was increased at 12 and 36 hours after TBI injury compare with control. Meanwhile, the pAdEasy-rNGB-GFP transfected rats suffered less necrosis and apoptosis compare to control.
Conclusions: NGB was upregulated in TBI and overexpressed rNGB had a significant neuroprotection in TBI. However, the mechanism remained unknown. This study suggested that rNGB overexpression may be a new strategy for treating of TBI.

• 出版日期2012-7
• 单位河北医科大学; 复旦大学; 中国人民解放军总医院