科研之友
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摘要
The aphid Schizaphis graminum is dependent on an association with Buchnera aphidicola, an eubacterial endosymbiont located in specialized host cells. Past studies have indicated that Escherichia coli is the closest known relative of the endosymbiont which has many genetic attributes of free-living bacteria. In order to obtain information on the properties of highly expressed genes, we have chosen for study the single-copy rrs (gene encoding 16S rRNA) of B. aphidicola. A 4.4-kb DNA fragment was cloned into E. coli and the nucleotide (nt) sequence determined. Several ORFs were identified; the order of genes was argS-rss-ORF1-rnh-dnaQ. ArgS, RNase H and DnaQ had 36-57% amino acid (aa) identity to the homologous proteins of E. coli. B. aphidicola rrs appears to be part of an operon consisting of a putative promoter, rrs and two inverted repeats resembling Rho-independent terminators. Comparisons of the sequences of argS-rrn DNA fragments from endosymbionts of six additional aphid species indicated conservation of sequences corresponding to a single - 35 (TTGACA) and - 10 (TGTAAT) promoter region, as well as boxA (sequence involved in antitermination) and boxC. The B. aphidicola argS-rrn DNA fragments from endosymbionts from seven species of aphids had promoter activities in E. coli which ranged from 6 tb 135% of that observed with a comparable DNA fragment of E. coli rrnB. Similarly, the putative B. aphidicola terminator was functional in E. coli. In most eubacteria, the rRNA-encoding genes are arranged in the order, 16S, 23S, 5S, and are part of a single operon. B. aphidicola differs from these organisms in that the gene encoding 16S rRNA. is on a transcription unit distinct from that for 23S and 5S rRNA.
- 出版日期1993-12-31
