A Functional NR4A Nuclear Receptor DNA-Binding Domain is Required for Organ Development in Caenorhabditis elegans

作者：Heard Melissa; Maina Claude V; Morehead Benjamin E; Hoener Marius C; Nguyen Tri Q; Williams Christopher C; Rowan Brian G; Gissendanner Chris R^*

来源：Genesis, 2010, 48(8): 485-491.

DOI：10.1002/dvg.20646

• NHR-6
• NR4A
• C. elegans
• DNA-binding domain
• spermatheca
• organogenesis

NR4A nuclear receptors are a diverse group of orphan nuclear receptors with critical roles in regulating cell proliferation and cell differentiation. The ortholog of the NR4A nuclear receptor in Caenorhabditis elegans, NHR-6, also has a role in cell proliferation and cell differentiation during organogenesis of the spermatheca. Here we show that NHR-6 is able to bind the canonical NR4A monomer response element and can transactivate from this site in mammalian HEK293 cells. Using a functional GFP-tagged NHR-6 fusion, we also demonstrate that NHR-6 is nuclear localized during development of the spermatheca. Mutation of the DNA-binding domain of NHR-6 abolishes its activity in genetic rescue assays, demonstrating a requirement for the DNA-binding domain. This study represents the first genetic demonstration of an in vivo requirement for an NR4A nuclear receptor DNA-binding domain in a whole organism. genesis 48:485-491,2010.

• 出版日期2010-8
• 单位New England Biolabs