A structural model for the N-terminal N1 module of E-coli glycogen branching enzyme

作者：Lo Leggio L^*; Ernst HA; Hilden I; Larsen S

来源：Biologia, 2002, 57: 109-118.

• glycogen branching enzyme
• N-domain
• alpha-amylase family
• domain duplication
• homology model
• structure

Prokaryotic branching enzymes can be divided into Group 1, with long N-domains, and Group 2, with short N-domains. Sequence analysis and fold-recognition approaches suggest that in Group 1 enzymes the N-domain has originated from duplication of a module with an immunoglobulin-type fold, similar in fold to the N-domain found in some other family 13 enzymes. These modules are here referred to as NI and N2. Group 2 enzymes only have one such module. A three-dimensional model of the N1 module of E. coli CBE, a Group 1 branching enzyme, has been constructed based on secondary structure alignment to the N-domains of Pseudomonas amyloderamosa isoamylase and Sulfolobus solfataricus glycosyltrehalose trehalohydrolase.

• 出版日期2002