Tomographic diffractive microscopy of living cells based on a common-path configuration

作者：Hsu Wei Chen; Su Jing Wei; Tseng Te Yu; Sung Kung Bin^*

来源：Optics Letters, 2014, 39(7): 2210-2213.

DOI：10.1364/OL.39.002210

We demonstrate a common-path tomographic diffractive microscopy technique for three-dimensional (3D) refractive-index (RI) imaging of unstained living cells. A diffraction grating is utilized to generate a reference beam that traverses a blank region of the sample in a common-path off-axis interferometry setup. Single-shot phase images captured at multiple illumination angles are used for 3D RI reconstruction based on optical diffraction tomography. The common-path configuration shows lower temporal phase fluctuations and better RI resolution than a Mach-Zehnder configuration. 3D subcellular RI distributions of live HeLa cells are quantified.

• 出版日期2014-4-1