Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)

作者：Okabe Hiroaki; Hui Shu Ping^*; Fuda Hirotoshi; Furukawa Takayuki; Takeda Seiji; Shrestha Rojeet; Miura Yusuke; Watanabe Mitsugu; Chiba Hitoshi

来源：Analytical Sciences, 2015, 31(12): 1341-1344.

DOI：10.2116/analsci.31.1341

• Amphipathicity
• polyphenol
• LC-MS/MS
• antioxidant
• Pacific oyster
• polar end-capped ODS

A novel amphipathic phenolic compound, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), that can be isolated from the Pacific oyster (Crassostrea gigas) has been found to protect human hepatocytes against oxidative stress. This study aims to establish a method for the measurement of DHMBA for industrial application. Liquid chromatography-tandem mass spectrometry using deuterated DHMBA as an internal standard and a polar end-capped ODS (Hypersil GOLD aQ) as the solid phase was validated. The limit of detection was 0.04 pmol (S/N = 5), and the limit of quantitation was 0.1 pmol (S/N = 10). The calibration curve was linear throughout the range of 0.1 - 16 pmol (r(2) = 0.9995). This method successfully quantified DHMBA in oysters from 11 sea areas in Japan. The results showed that the yield of DHMBA was variable from 9.8 to 58.8 mu g g(-1) whole oyster meat wet weight but not affected by the seawater temperature. The proposed LC-MS/MS method is useful in quantitative studies for DHMBA and potentially for other amphipathic substances.

• 出版日期2015-12