Development and characterization of twenty-five microsatellite markers for the longnose dace (Cyprinidae: Rhinichthys) using paired-end Illumina shotgun sequencing

作者：Beasley Rochelle; Lance Stacey L; Ruskey Jennifer A; Taylor Eric B^*

来源：Conservation Genetics Resources, 2014, 6(4): 1011-1013.

DOI：10.1007/s12686-014-0272-y

• Rhinichthys
• Microsatellite
• PAL_FINDER
• PCR primers
• SSR

We isolated and characterized a total of 48 microsatellite loci in Rhinichthys cataractae a widely distributed freshwater fish that may contain several cryptic species. Loci were screened in 24 individuals from several areas of British Columbia, Canada. The number of alleles per locus ranged from 5 to 24, observed heterozygosity ranged from 0.250 to 0.940, and the probability of identity ranged from 0.006 to 0.453. These new loci are being used for conducting investigations into the genetic structure, diversity, and speciation in populations of this exceptionally broadly-distributed North American freshwater fish.

• 出版日期2014-12