Distribution of HLA-A, -B, and -C Alleles and HLA/KIR Combinations in Han Population in China

作者：Shen, Yunsong; Cao, Danfeng; Li, Yalin; Kulski, J. K.; Shi, Lei; Jiang, Hongjun; Ma, Qianli; Yu, Jiankun; Zhou, Jingxian; Yao, Yufeng^*; Shi, Li

来源：Journal of Immunology Research, 2014, 2014: 565296.

DOI：10.1155/2014/565296

• IMMUNOGLOBULIN-LIKE RECEPTOR
• KIR GENES
• SOUTHWEST CHINA
• YUNNAN PROVINCE
• KILLER
• HAPLOTYPES
• DIVERSITY
• POLYMORPHISM
• EXPRESSION
• MOLECULES

We investigated polymorphisms of the human leukocyte antigen (HLA) class I (A, B, and C) loci of a Han population (n, 239) from the Yunnan province, Southwest China, using high-resolution polymerase chain reaction-Luminex (PCR-Luminex) typing. We combined the HLA data from this study with the KIR genotypes from a previous study of this Han population to analyze the combination of KIR/HLA ligands. A total of 27 HLA-A, 54 HLA-B, and 31 HLA-C alleles were found in this population. The frequencies of A*11:01, A*24: 02, B*40:01, B*46:01, C*01:02, C*03:04, and C*07:02 were all > 10%. The following haplotypes were common, with frequencies > 5%: 1 A-B (A*02:07-B*46:01), 2 A-C (A*02:07-C*01:02, and A*11:01-C*07:02), 4 C-B (B*13:01-C*03:04, B*40:01-C*07:02, B*46:01-C*01:02 and B*58: 01-C*03:02), and 1 A-C-B (A*02:07-C*01:02-B*46:01). Analysis of KIR3D and their ligands HLA-A3/A11 and HLA-Bw4 showed that the frequencies of 3DL2(+)-A3/A11(+) and 3DL2(+)-A3/A11(-) were 0.527 and 0.473, and the frequencies of 3DL1(+)-Bw4(+), 3DL1(+)-Bw4(-), 3DL1-Bw4(+), and 3DL1-Bw4(-) were 0.552, 0.397, 0.038, and 0.013, respectively. The results of KIR/HLA-C combination analysis showed that all individuals had at least one inhibitory or activating KIR/HLA-C pair, and one KIR/HLA-Cpairwas the most frequent (157/239), followed by two pairs (46/239), three pairs (33/239), and no pairs (3/239). Comparison of KIR gene and HLA gene and their pair frequency between Yunnan Han and the isolated Han (FYDH) who also lived in Yunnan province showed no significant difference (P > 0.05) in KIR frequencies, but significant differences (P < 0.05) for some HLA allele frequencies. In addition, there was no significant difference (P > 0.05) between the two populations for KIR/HLA pairs.

• 出版日期2014
• 单位中国医学科学院医学生物学研究所; 昆明医科大学; 中国医学科学院北京协和医院; 昆明理工大学