Monitoring Tumor Response After Histone Deacetylase Inhibitor Treatment Using 3'-Deoxy-3'-[F-18]-fluorothymidine PET

作者：Chan Pei Chia; Wu Chun Yi; Chou Lin Shan; Ho Chung Hsien; Chang Chi Wei; Chiou Shih Hwa; Lin Wuu Jyh; Chen Fu Du; Chang C Allen; Hwang Jeng Jong; Liu Ren Shyan^*; Wang Hsin Ell

来源：Molecular Imaging and Biology, 2015, 17(3): 394-402.

DOI：10.1007/s11307-014-0774-8

• [F-18]FLT
• HDACi
• Treatment response
• MicroPET
• Hepatocellular carcinoma

This study employed 3'-deoxy-3'-[F-18]-fluorothymidine ([F-18]FLT) microPET scanning to assess the treatment response of histone deacetylase inhibitors (HDACi), e.g., N1-hydroxy-N8-phenyloctanediamide (SAHA) and its iodinated derivative ISAHA, in a hepatoma mouse model.
The in vitro cytotoxicity of HDACi in various hepatoma cell lines was determined by MTT assay and flow cytometry. ISAHA and SAHA were used to treat HepG2 hepatoma xenograft-bearing mice. The treatment responses were characterized in terms of tumor burden, microPET imaging, and immunohistochemical staining of tumor sections.
ISAHA effectively inhibited HepG2 hepatoma cell survival and tumor growth. A significantly reduced tumor uptake during HDACi treatment was noticed in [F-18]FLT microPET imaging, which was consistent with the findings in immunohistochemical staining.
ISAHA can suppress tumor cell proliferation both in vitro and in vivo. [F-18]FLT PET is a promising modality for evaluating the in vivo therapeutic efficacy of HDACi at the early stage of treatment.

• 出版日期2015-6
• 单位中国医科大学