Proteomic Approach to the Study of Statin Pleiotropy in Kidney Transplant Patients

作者：Perez Vanessa; Navarro Munoz Maribel; Mas Sebastian; Bayes Beatriz; Cruz Pastor Mari; Martinez Caceres Eva; Lauzurica Ricardo; Egido Jesus; Romero Ramon^*

来源：Pharmacology, 2011, 87(3-4): 161-168.

DOI：10.1159/000324311

• Statins
• Kidney transplantation
• Proteomics
• Magnetic beads
• Matrix-assisted laser desorption/ionization time-of-flight
• Bradykinin
• Complement factor C4

Background/Aims: Statins are prescribed in kidney transplant recipients in order to manage dyslipidemia, a common complication in these patients. The efficacy of statins in reducing cholesterol levels has been accompanied by pleiotropic effects. Fifty-four kidney transplant patients were included in the present study, the objective of which was to ascertain the effect of 12 weeks of atorvastatin therapy (10 mg/day) on the patients' lipid profile, renal function, markers of inflammation and plasma peptide profile. Methods: Biochemical variables were determined with a routine clinical laboratory analyzer, and the proteomic approach was based on magnetic particle-assisted sample processing coupled to mass spectrometry readout. Results: Atorvastatin therapy improved the lipid profile of patients and caused significant changes in their plasma peptide profile; peptides with m/z 1063 and 1398 decreased after treatment and were identified as fragments derived from molecules involved in vascular inflammation, i.e. high-molecular-weight kininogen and complement factor C4, respectively. Conclusion: These findings may contribute to the growing body of evidence of the anti-inflammatory actions attributed to statins, by which these drugs could improve these patients' clinical status.

• 出版日期2011