A rapid mismatch polymerase chain reaction assay to detect carriers of complex vertebral malformation in Holstein cattle

作者：Wang, Shuai; Hao, Haisheng; Zhao, Xueming; Zhu, Huabin; Du, Weihua; Wang, Dong; Liu, Yan; Qin, Tong; Wang, Zongli^*

来源：Journal of Veterinary Diagnostic Investigation, 2012, 24(3): 568-571.

DOI：10.1177/1040638712441605

• Cattle
• complex vertebral malformation
• polymerase chain reaction mismatch amplification mutation assay
• polymerase chain reaction-primer introduced restriction analysis

Three Holstein sires were identified as complex vertebral malformation (CVM) carriers using the polymerase chain reaction-primer introduced restriction analysis (PCR-PIRA) method. Using the carriers as positive controls, the PCR mismatch amplification mutation assay (MAMA PCR) method was developed and validated by sequence analysis. With MAMA PCR, 154 Chinese Holstein sires were tested for CVM, among which 24 were confirmed to be CVM carriers. With DNA isolated from hair follicles, 10 daughters of one CVM-positive bull were detected, among which 7 were confirmed to be CVM carriers.

• 出版日期2012-5
• 单位中国农业科学院; 甘肃农业大学