A vesicular glutamate transporter in lampreys: cDNA cloning and early expression in the nervous system

作者：Villar Cervino Verona; Rocancourt Claire; Menuet Arnaud; Da Silva Corinne; Wincker Patrick; Anadon Ramon; Mazan Sylvie; Celina Rodicio Maria

来源：Journal of Chemical Neuroanatomy, 2010, 40(1): 71-81.

DOI：10.1016/j.jchemneu.2010.03.009

• Glutamate transporter gene
• VGLUT
• In situ hybridization
• Brain
• Cranial nerve ganglia
• Early development
• Petromyzon marinus L.

Vesicular glutamate transporters (VGLUTs) accumulate glutamate into synaptic vesicles of glutamatergic neurons, and thus are considered to define the phenotype of these neurons. Glutamate also appears to play a role in the development of the nervous system of vertebrates. Here we report the characterization of a vesicular glutamate transporter of lamprey (IVGluT), a novel member of the VGluT gene family. Phylogenetic analysis indicates that IVGLUT cannot be assigned to any of the three VGLUT isoforms characterized in teleosts and mammals, suggesting that these classes may have been fixed after the splitting between cyclostomes and gnathostomes. Expression pattern analysis during lamprey embryogenesis and prolarval stages shows that IVGluT expression is restricted to the nervous system. The first structure to express IVGluT was the olfactory epithelium of late embryos. In the brain of early prolarvae, IVGluT was expressed in most of the neuronal populations that generate the early axonal scaffold. IVGluT expression was also observed in neuronal populations of the rhombencephalon and spinal cord and in ganglia of the branchiomeric, octaval and posterior lateral line nerves. In the rhombencephalon, IVGluT expression appears to be spatially restricted in dorsal and ventral longitudinal domains. Comparison of the early expression of VGluT genes between the lamprey and some anamniotan gnathostomes (frog, zebrafish) reveals a conserved expression pattern, likely to reflect ancestral vertebrate characteristics.

• 出版日期2010-9
• 单位中国地震局