Abrogation of the Twin Arginine Transport System in Salmonella enterica Serovar Typhimurium Leads to Colonization Defects during Infection

作者：Reynolds M Megan^*; Bogomolnaya Lydia; Guo Jinbai; Aldrich Lindsay; Bokhari Danial; Santiviago Carlos A; McClelland Michael; Andrews Polymenis Helene

来源：PLos One, 2011, 6(1): e15800.

DOI：10.1371/journal.pone.0015800

TatC (STM3975) is a highly conserved component of the Twin Arginine Transport (Tat) systems that is required for transport of folded proteins across the inner membrane in gram-negative bacteria. We previously identified a Delta tatC mutant as defective in competitive infections with wild type ATCC14028 during systemic infection of Salmonella-susceptible BALB/c mice. Here we confirm these results and show that the Delta tatC mutant is internalized poorly by cultured J774-A.1 mouse macrophages a phenotype that may be related to the systemic infection defect. This mutant is also defective for short-term intestinal and systemic colonization after oral infection of BALB/c mice and is shed in reduced numbers in feces from orally infected Salmonella-resistant (CBA/J) mice. We show that the Delta tatC mutant is highly sensitive to bile acids perhaps resulting in the defect in intestinal infection that we observe. Finally, the Delta tatC mutant has an unusual combination of motility phenotypes in Salmonella; it is severely defective for swimming motility but is able to swarm well. The Delta tatC mutant has a lower amount of flagellin on the bacterial surface during swimming motility but normal levels under swarming conditions.

• 出版日期2011-1-26