Processing of the Escherichia coli leuX tRNA transcript, encoding tRNA(Leu5), requires either the 3'5' exoribonuclease polynucleotide phosphorylase or RNase P to remove the Rho-independent transcription terminator

作者：Mohanty Bijoy K; Kushner Sidney R^*

来源：Nucleic Acids Research, 2010, 38(2): 597-607.

DOI：10.1093/nar/gkp997

Here we report a unique processing pathway in Escherichia coli for tRNA(Leu5) in which the exoribonuclease polynucleotide phosphorylase (PNPase) removes the Rho-independent transcription terminator from the leuX transcript without requiring the RhlB RNA helicase. Our data demonstrate for the first time that PNPase can efficiently degrade an RNA substrate containing secondary structures in vivo. Furthermore, RNase P, an endoribonuclease that normally generates the mature 5'-ends of tRNAs, removes the leuX terminator inefficiently independent of PNPase activity. RNase P cleaves 4-7 nt downstream of the CCA determinant generating a substrate for RNase II, which removes an additional 3-4 nt. Subsequently, RNase T completes the 3' maturation process by removing the remaining 1-3 nt downstream of the CCA determinant. RNase E, G and Z are not involved in terminator removal. These results provide further evidence that the E. coli tRNA processing machinery is far more diverse than previously envisioned.

• 出版日期2010-1