High-fat meals induce systemic cytokine release without evidence of endotoxemia-mediated cytokine production from circulating monocytes or myeloid dendritic cells

作者：Fogarty Christopher L; Nieminen Janne K; Peraneva Lina; Lassenius Mariann I; Ahola Aila J; Taskinen Marja Riitta; Jauhiainen Matti; Kirveskari Juha; Pussinen Pirkko; Horkko Sohvi; Makinen Ville Petteri; Gordin Daniel; For**lom Carol; Groop Per Henrik; Vaarala Outi; Lehto Markku^*

来源：ACTA DIABETOLOGICA, 2015, 52(2): 315-322.

DOI：10.1007/s00592-014-0641-8

• Type 1 diabetes
• High-fat diet
• Inflammation
• Lipopolysaccharide
• Endotoxin
• Cytokines

Dietary fats have been shown to promote the translocation of bacterial endotoxins from the gut into circulation, which may induce systemic inflammation and modulate the inflammatory response of circulating immune cells. The aim of this study was to determine the effect of the postprandial milieu on inflammation and the inflammatory response of antigen presenting cells in the context of type 1 diabetes (T1D).
Eleven patients with T1D and eleven nondiabetic controls were recruited as part of the FinnDiane study and given two fatty meals during 1 day. Cytokine responses in monocytes and myeloid dendritic cells (mDCs) as well as serum lipopolysaccharide activity levels, triglyceride concentrations and cytokine concentrations were measured from fasting and postprandial blood samples.
Postprandially, patients with T1D and controls showed significant increases in eight inflammatory cytokines (IL-6, TNF-alpha, IL-1 beta, IFN-alpha, IL-10, IFN-gamma, IL-12 and MIP-1 beta) without concomitant increase in serum LPS activity. Serum cytokine production was similar in both groups. No postprandial change was seen in the IL-6, TNF-alpha or IL-1 beta production of mDCs or monocytes. At fasting, diabetic mDCs exhibited higher LPS-induced IL-6 and IL-1 beta production than controls.
Acute high-fat meals increase circulating cytokines but have no effect on serum lipopolysaccharide activity levels or cytokine production in circulating mDCs or monocytes. Our results suggest that postprandial increase in serum cytokine levels is neither mediated by circulating endotoxins nor the activation of circulating innate cells. The production of high-fat meal-induced inflammatory markers is most likely regulated at the tissue level.

• 出版日期2015-4