Southern blot screening for lignin peroxidase and aryl-alcohol oxidase genes in 30 fungal species

作者：Varela E; Martinez AT; Martinez MJ^*

来源：Journal of Biotechnology, 2000, 83(3): 245-251.

DOI：10.1016/S0168-1656(00)00323-0

• white-rot fungi
• southern-blot
• ligninolytic enzymes
• gene screening

Screening to detect genes encoding lignin peroxidase (LiP) and aryl-alcohol oxidase (AAO) has been carried out with 30 fungal strain using DNA probes from genes lpo of Phanerochaete chrysosporium (encoding LIP isoenzyme H8) and acro of Pleurotus eryngii. Evidence for the presence of genes closely related to lpo was found in Bjerkandera adusta Fomes fomentarius, Ganoderma applanatum, Ganoderma australe, Lentinula degener, Peniophora gigantea, P. chrysosporium, Phanerochaete flavido-alba and Trametes versicolor, whereas the gene ano was detected in Pleurotus species and B. adusta. The presence of both genes was only detected in B. adusta. These results suggest that different enzymatic system, formed by enzymes encoded by different genes, are responsible for lignin degradation by white-rot fungi.

• 出版日期2000-10-13