Molecular Proteomics Imaging of Tumor Interfaces by Mass Spectrometry

作者：Kang Suki; Shim Hyo Sup; Lee Jong Sik; Kim Dong Su; Kim Hak Yong; Hong Seong Hyun; Kim Pan Soo; Yoon Joo Heon; Cho Nam Hoon^*

来源：Journal of Proteome Research, 2010, 9(2): 1157-1164.

DOI：10.1021/pr900666q

• MALDI-IMS
• interface
• 2-dimensional gel electrophoresis
• ovarian cancer

The specific molecular profiles of ovarian cancer interface zones (2), the region between tumors and normal tissues, were evaluated using a new method involving matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). We analyzed three ovarian serous carcinomas using MALDI-IMS. Principal component analysis (PCA) was used to evaluate the quality of tissue spatial features based on MALDI-IMS, and for analysis of large data sets of MALDI-IMS. Two-dimensional gel electrophoresis and fluorescence microscopy were used to verify interface-specific proteins. Unique profiles were identified for the tumors, the normal zone, and the IZ. Through MALDI analysis, two interfacespecific proteins, plastin 2 and peroxiredoxin 1 (PRDX 1), were identified as differentially regulated between zones. Fluorescence microscopy revealed high expression levels of plastin 2 and PRDX 1 along the IZ of ovarian tumors. This comparative proteomics study using tissue MALDI-IMS suggested that the IZ is different from the adjacent tumor and normal zones, and that plastin 2 and PRDX 1 may be interface markers specific to ovarian tumors.

• 出版日期2010-2