Novel O-antigen of Hafnia alvei PCM 1195 lipopolysaccharide with a teichoic acid-like structure

作者：Niedziela Tomasz^*; Kenne Lennart; Lugowski Czeslaw

来源：Carbohydrate Research, 2010, 345(2): 270-274.

DOI：10.1016/j.carres.2009.11.005

• Hafnia alvei
• O-antigen
• Lipopolysaccharide
• Phosphodiester
• NMR spectroscopy
• MALDI-TOF mass spectrometry

The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by H-1, C-13, and P-31 NMR spectroscopy, MALDI-TOF MS, and GC-MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: {-> 4-alpha-D-Glcp-(1 -> 3)-alpha-L-FucpNAc-(1 -> 3)-[alpha-D-Glcp-(1 -> 4)]-alpha-D-GlcpNAc-(1 -> 3)-alpha-L-FucpNAc-(1 -> 4)-alpha-D-Glcp-(1 -> P}(n).

• 出版日期2010-1-26