Adipose-derived stem cells sustain prolonged angiogenesis through leptin secretion

作者：Delle Monache Simona^*; Calgani Alessia; Sanita Patrizia; Zazzeroni Francesca; Warschauer Emilio Gentile; Giuliani Antonio; Amicucci Gianfranco; Angelucci Adriano

来源：Growth Factors, 2016, 34(3-4): 87-96.

DOI：10.1080/08977194.2016.1191481

• Angiogenesis
• growth factors
• endothelial progenitor cells
• CD105

Recent studies suggest that adipose-derived stem cells (ASCs) play a role in tissue remodeling through the release of cytokines and growth factors. We compared the secreted cytokine profile of hypoxia-conditioned ASCs (hASCs) with normoxic ASCs (nASCs) and we analyzed the effect of ASCs conditioned medium (CM) on endothelial cells. We found that hypoxia induced a transient upregulation of VEGF in ASCs and a notable and enduring upregulation of leptin mRNA expression 30-fold greater than control after 24 h and up to 60-fold greater than control at day 7. CM from hASC stimulated EC tube formation to a significantly greater extent than CM from nASC. This might be due to leptin-secreted factor. Indeed, exogenous leptin stimulated the expression of HIF2-alpha, but not HIF1-alpha, and upregulated the expression of Flt-1 and Tie-1 proangiogenic receptors. In conclusion, hASCs may be particularly efficient in sustaining angiogenesis through the release of leptin.

• 出版日期2016-8