Fast Separation and Determination of Sterols in Vegetable Oils by Ultraperformance Liquid Chromatography with Atmospheric Pressure Chemical Ionization Mass Spectrometry Detection

作者：Jesus Lerma Garcia Maria; Francisco Simo Alfonso Ernesto; Mendez Alberto; Lluis Lliberia Josep; Manuel Herrero Martinez Jose

来源：Journal of Agricultural and Food Chemistry, 2010, 58(5): 2771-2776.

DOI：10.1021/jf9043887

• Botanical origin
• mass spectrometry
• sterols
• ultraperformance liquid chromatography
• vegetable oils

A method for the determination of sterols in vegetable oils by ultraperformance liquid chromatography (UPLC) with atmospheric pressure chemical ionization mass spectrometry detection has been developed. The separation of sterols was optimized in terms of mobile phase composition, column temperature and flow rate. The optimal conditions were achieved using an Acquity UPLC BEH C18 column (50 x 2.1 mm, 1.7 mu m) with a mobile phase consistent of acetonitrile/water (0.01% acetic acid) using a linear gradient, at a flow rate of 0.8 mL min(-1) and column temperature of 10 degrees C, giving a total analysis time below 5 min. The determination was performed in selective ion recording mode. The limits of detection were in all cases below 0.07 mu g mL(-1), with relative standard deviation values of retention times and peak areas below 0.4 and 5%, respectively. The content of main sterols present in several vegetable oils with different botanical origins was also established.

• 出版日期2010-3-10