A COMPARATIVE ANALYSIS OF THREE METHODS USED FOR RNA QUANTITATION

作者：Schipor Sorina^*; Vladoiu Suzana; Baciu Ancuta Elena; Niculescu Ana Maria; Caragheorgheopol Andra; Iancu Iulia; Plesa Adriana; Popescu A I; Manda Dana

来源：Romanian Reports in Physics, 2016, 68(3): 1178-1188.

• RNA quantitation
• UV spectrophotometry
• spectrophotofluorimetry
• microfluidic capillary electrophoresis

The high RNA sample quality is essential for downstream molecular biology applications. Two simultaneous conditions should be accomplished by the RNA samples: the structural integrity of the molecules and an adequate concentration. The objective of this study is to do a comparative analysis between three different methods of measuring RNA concentration. The three methods considered here are: UV spectrophotometry, spectrophotofluorimetry, and microfluidic capillary electrophoresis. Sixteen RNA samples were assayed by these-three methods. The principles and the results of each method as well as advantages, disadvantages and perturbations factors are analyzed and discussed. Fluorescent labelling of RNA gives more accurate results even in the presence of the frequent contaminants like DNA and proteins. Knowing the strength and the limits of each method required for RNA quantitation, the scientist can choose the most cost-effective protocol.

• 出版日期2016