Ultrasonic-Assisted Extraction and Chromatography Separation of Polysaccharides from the Base of Flammulina velutipes Stipe

作者：Zhao, Liyan; Fu, Yaxin; Chen, Chen; Yang, Wenjian; Hu, Qiuhui^*

来源：Separation Science and Technology (Philadelphia), 2015, 50(6): 824-832.

DOI：10.1080/01496395.2014.964728

• base of Flammulina velutipes stipe
• ultrasonic extraction
• chromatography separation
• response surface methodology
• polysaccharides

Ultrasonic-assisted extraction (UAE) and response surface methodology (RSM) were applied to the extraction of polysaccharides from the base of Flammulina velutipes stipe. Results showed that the optimum extraction parameters were ultrasonic power 680 W, time 19.8 min, the ratio of water to raw material 28 mL/g, and the yield of polysaccharides was 16.20%. After separation by DEAE cellulose-52 and Sephadex G-100 size-exclusion chromatography, three main fractions, FVSP -1, FVSP -2, and FVSP -3, were obtained. The relative molecular weights of which were 21 kDa, 224 kDa, 357 kDa, respectively, and all the three fractions were heteropolysaccharides with a glucan as backbone chain.

• 出版日期2015-4-13
• 单位南京农业大学; 南京财经大学