A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting

作者：Palumbo Fabio Salvatore; Volpe Antonella Bavuso; Bongiovi Flavia; Pitarresi Giovanna^*; Giammona Gaetano

来源：Macromolecular Bioscience, 2015, 15(11): 1605-1615.

DOI：10.1002/mabi.201500129

• ATRP
• CD44
• hyaluronic acid
• siRNA delivery
• tumor targeting

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.

• 出版日期2015-11