Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses

作者：Dvorakova Vera; Bartenschlager Heinz; Stratil Antonin^*; Horak Pavel; Stupka Roman; Citek Jaroslav; Sprysl Michal; Hrdlicova Anna; Geldermann Hermann

来源：Genetics Selection Evolution, 2012, 44: 13.

DOI：10.1186/1297-9686-44-13

Background: Independent studies have shown that several single nucleotide polymorphisms (SNP) in the human FTO (fat mass and obesity associated) gene are associated with obesity. SNP have also been identified in the pig FTO gene, among which some are associated with selected fat-deposition traits in F-2 crosses and commercial populations. In this study, using both commercial pig populations and an experimental Meishan x Pietrain F-2 population, we have investigated the association between one FTO SNP and several growth and carcass traits. Association analyses were performed with the FTO polymorphism either alone or in combination with polymorphisms in flanking loci. %26lt;br%26gt;Methods: SNP (FM244720:g.400C%26gt;G) in exon 3 of porcine FTO was genotyped by PCR-RFLP and tested for associations with some growth, carcass and fat-related traits. Proportions of genetic variance of four pig chromosome 6 genes (FTO, RYR1, LIPE and TGFB1) on selected traits were evaluated using single-and multi-locus models. %26lt;br%26gt;Results: Linkage analysis placed FTO on the p arm of pig chromosome 6, approximately 22 cM from RYR1. In the commercial populations, allele C of the FTO SNP was significantly associated with back fat depth and allele G with muscling traits. In the Meishan x Pietrain F-2 pigs, heterozygotes with allele C from the Pietrain sows and allele G from the Meishan boar were more significantly associated with fat-related traits compared to homozygotes with allele G from the Pietrain and allele G from the Meishan breed. In single-and multi-locus models, genes RYR1, TGFB1 and FTO showed high associations. The contribution in genetic variance from the polymorphism in the FTO gene was highest for back fat depth, meat area on the musculus longissimus lumborum et thoracis tissues and metabolite glucose-6-phosphate dehydrogenase. %26lt;br%26gt;Conclusions: Our results show that in pig, FTO influences back fat depth in the commercial populations, while in the Meishan x Pietrain F-2 pigs with a CG genotype, heterosis occurs for several fat-related traits.

• 出版日期2012-4-17