A novel indole alkaloid from deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth

作者：Chen Lin; Tang Xi Xiang; Zheng Min; Yi Zhi Wei; Xiao Xiang; Qiu Ying Kun^*; Wu Zhen

来源：Journal of Asian Natural Products Research, 2011, 13(5): 444-448.

DOI：10.1080/10286020.2011.570262

• deep-sea sediment
• metagenomic clone
• N-{1-[4-(acetylamino)phenyl]-3-hydroxy-1-(1H-indol-3-yl)propan-2-yl}-2
• 2-dichloroacetamide
• indole alkaloids

To explore secondary metabolites in deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth, different kinds of chromatography methods were used in the isolation procedures, while the structures of the isolated compounds were assigned based on the MS analysis and their 1H and 13C NMR spectra including 2D NMR techniques such as COSY, HMQC, and HMBC experiments. As a result, a novel compound was isolated and characterized as N-{1-[4-(acetylamino)phenyl]-3-hydroxy-1-(1H-indol-3-yl)propan-2-yl}-2,2-dichloroacetamide (1). In addition, eight known compounds were also obtained. Fatty acid amide hydrolase and monoacylglycerol lipase were used to screen analgesic activity, and the new compound showed analgesic activity to some extent in pharmacological test.

• 出版日期2011
• 单位厦门大学; 国家海洋局第三海洋研究所