Stem cell fate dictated solely by altered nanotube dimension

作者：Oh Seunghan; Brammer Karla S; Li Y S Julie; Teng Dayu; Engler Adam J; Chien Shu; Jin Sungho^*

来源：Proceedings of the National Academy of Sciences, 2009, 106(7): 2130-2135.

DOI：10.1073/pnas.0813200106

• differentiation
• mesenchymal
• nanotopography
• osteogenesis
• proliferation

Two important goals in stem cell research are to control the cell proliferation without differentiation and to direct the differentiation into a specific cell lineage when desired. Here, we demonstrate such paths by controlling only the nanotopography of culture substrates. Altering the dimensions of nanotubular-shaped titanium oxide surface structures independently allowed either augmented human mesenchymal stem cell (hMSC) adhesion or a specific differentiation of hMSCs into osteoblasts by using only the geometric cues, absent of osteogenic inducing media. hMSC behavior in response to defined nanotube sizes revealed a very dramatic change in hMSC behavior in a relatively narrow range of nanotube dimensions. Small (approximate to 30-nm diameter) nanotubes promoted adhesion without noticeable differentiation, whereas larger (approximate to 70- to 100-nm diameter) nanotubes elicited a dramatic stem cell elongation (approximate to 10-fold increased), which induced cytoskeletal stress and selective differentiation into osteoblast-like cells, offering a promising nanotechnology-based route for unique orthopedics-related hMSC treatments.

• 出版日期2009-2-17