Bombesin functionalized Cu-64-copper sulfide nanoparticles for targeted imaging of orthotopic prostate cancer

作者：Cai, Huawei^*; Xie, Fang; Mulgaonkar, Aditi; Chen, Lihong; Sun, Xiankai; Hsieh, Jer-Tsong; Peng, Fangyu; Tian, Rong; li, Lin; Wu, Changqiang^*; Ai, Hua

来源：Nanomedicine, 2018, 13(14): 1695-1705.

DOI：10.2217/nnm-2018-0062

• bombesin
• Cu-64-copper sulfide nanoparticles
• micro-PET-CT
• prostate cancer

Aim: To synthesize and evaluate the imaging potential of Bom-PEG-[Cu-64] CuS nanoparticles (NPs) in orothotopic prostate tumor. Materials & methods: [Cu-64] CuS NPs were synthesized in aqueous solution by (CuCl2)-Cu-64 and Na2S reaction. Then PEG linker with or without bombesin peptide were conjugated to the surface of [Cu-64] CuS NPs to produce Bom-PEG-[Cu-64] CuS and PEG-[Cu-64] CuS NPs. These two kinds of NPs were used for testing specific uptake in prostate cancer cells in vitro and imaging of orthotopic prostate tumor in vivo. Results: Bom-PEG-[Cu-64] CuS and PEG-[Cu-64] CuS NPs were successfully synthesized with core diameter of approximately 5 nm. Radioactive cellular uptake revealed that Bom-PEG-[Cu-64] CuS was able to specifically bind to prostate cancer cells, and the microPET-CT imaging indicated clear visualization of orthotopic prostate tumors. Conclusion: Radiolabeled Bom-PEG-[Cu-64] CuS NPs have potential as an ideal agent for orthotopic prostate tumor imaging by microPET-CT.

• 出版日期2018-7
• 单位四川大学; 复旦大学